A new model of post-stroke depression established by ischemia/reperfusion combined with unpredictable stress

doi:10.3969/j.issn.2095-4344.1107

Abstract

Abstract:

BACKGROUND: Animal models of post-stroke depression are established by stroke combined with depression induction, and each modeling method has its own advantages and shortcomings. A new modeling method is needed.
OBJECTIVE: To explore a stable and replicable model of post-stroke depression through behavioral assessment and brain tissue neurotransmitter detection.
METHODS: Ninety male Sprague-Dawley rats weighting (200±20) g provided by Laboratory Animal Center of Xi’an Jiaotong University were enrolled. Sixty rats were randomly divided into three groups and then underwent 30, 60 and 90 minutes of ischemia/reperfusion. The survival rate of rats was observed after 24 hours, and the scores of neurological function deficit and the volume of cerebral infarct were evaluated. The optimal time points of ischemia/reperfusion were determined. The remaining 30 rats were randomly divided into sham, middle cerebral artery occlusion, and post-stroke depression groups. Forced swimming test, open field test, the novelty-suppressed feeding test, sucrose preference test and body mass change were used to evaluate rat depression behaviors before and 1, 2, and 3 weeks after stimulation. The levels of 5-hydroxytryptamine, norepinephrine, and brain-derived neurotrophic factor in brain tissue were detected by ELISA.
RESULTS AND CONCLUSION: The optimal time point 60 minutes was determined for subsequent ischemia/reperfusion. Behavioral evaluation showed that compared with the middle cerebral artery occlusion group, body mass, sucrose preference test and the vertical and horizontal movement scores of the open field test began to decrease at 2 weeks in the post-stroke depression group (P < 0.05). The latency of the novelty-suppressed feeding and the time of forced swimming test were significantly increased (P < 0.05). The results of ELISA showed that 5-hydroxytryptamine, norepinephrine, and brain-derived neurotrophic factor levels in the post-stroke depression group were significantly lower than those in the middle cerebral artery occlusion group (P < 0.05). Our findings imply that the post-stroke depression model can be established by 60-minute ischemia/reperfusion combined with chronic unpredictable mild stress. The models are stable, have a high survival rate and many clinical characteristics of post-stroke depression. It may become a new and reliable model for the study of post-stroke depression.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Stroke, Infarction, Middle Cerebral Artery, Tissue Engineering

CLC Number:

R466，R318

Zhao Bingbing, Li Tao, Wang Dou, Zhou Qi, Yan Yongmei. A new model of post-stroke depression established by ischemia/reperfusion combined with unpredictable stress[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.1107.

Figures/Tables 3

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